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Canine Coccidiodes immitis IgM ELISA KIT |
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Specifications |
48T/96T |
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Species |
dog |
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Sample type |
serum, plasma, tissue homogenate,cell lysate, cell Culture supernatant etc. |
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Detection range |
See the manual for details |
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Sensitivity |
See the manual for details |
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Response time |
1-5h |
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Sample volume |
10ul |
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Detection wavelength |
450nm |
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Delivery date |
3-5 days |
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Manual |
Consult our salesperson for obtaining |
Precision
The precision is expressed by the coefficient of variation CV of the measured value of the sample. CV (%) = SD/mean×100
Intra-batch difference: Take the same batch of kits for quantitative detection of low, medium, and high value fixed value samples. Each sample is measured 20 times continuously, and the average value and SD value of samples of different concentrations are calculated.
Inter-batch difference: select 3 different batches of kits to quantitatively determine the low, medium, and high-value fixed value samples. Use the same kit for each sample to repeat the measurement 10 times, and calculate the average value and SD of samples with different concentrations. value.
Intra-run difference: CV<4.7%
Difference between batches: CV<6.5%
Stability
It has been determined that the kit must be stored at the recommended temperature within the validity period, and the activity reduction rate is less than 5%. In order to reduce the influence of external factors on the detection value before and after the destruction of the kit, the environmental conditions of the laboratory should be kept as consistent as possible, especially the temperature, humidity and incubation conditions in the laboratory. Secondly, the operation by the same experimenter can reduce human error.
Kit composition
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Serial number |
English name |
specification |
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1 |
Wash solution(30X) |
20ml×1 bottle |
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2 |
HRP-Conjugate reagent |
6ml×1 bottle |
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3 |
Microelisa stripplate |
12 holes×8 strips |
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4 |
Sample diluent |
6ml×1 bottle |
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5 |
Chromogen Solution A |
6ml×1 bottle |
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6 |
Chromogen Solution B |
6ml×1/bottle |
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7 |
Stop solution |
6ml×1 bottle |
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8 |
Standard |
0.5ml×1 bottle |
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9 |
Standard diluent |
1.5ml×1 bottle |
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10 |
Product Description |
1 serving |
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11 |
Sealing plate membrane |
2 sheets |
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12 |
Sealing bag |
1 piece |
Note: Please check whether the label and quantity of the reagents in the kit are consistent with the table before use.
1. Microplate reader (it is recommended to warm up the instrument before use)
2. Precise pipettes, tips, and sample tanks
3. Distilled or deionized water
4. Bottle washing or automatic plate washing machine
5. 37℃ water bath or thermostat
6. EP tube
7. Powder-free disposable latex gloves
Storage conditions and validity period
1. Store at 2-8℃, do not freeze, the validity period is 6 months.
2. After opening and use, put the coated microplate into a ziplock bag with desiccant, seal the ziplock bag, and put all reagents back into the refrigerator at 2-8°C.




