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Fatty Acid Synthase (FAS) Assay Kit (Spectrophotometry/24 samples)

Item No.: 00472
Name: Fatty Acid Synthase (FAS) Assay Kit
Measurement method: Spectrophotometric method
Specification: 25 tubes/24 samples

Storage condition: low temperature and keep away from light
Validity: 6 months
Description
Description

Name: Fatty Acid Synthase (FAS) Assay Kit

Measurement method: Spectrophotometric method

Specification: 25 tubes/24 samples

Storage condition: low temperature and keep away from light

Validity: 6 months

Note: Choose 2-3 samples with large expected differences for pre-calibration before formal determination.

 

Measurement significance:

FAS is a key enzyme in fatty acid synthesis, catalysing the production of long-chain fatty acids from acetyl coenzyme A and malonyl coenzyme A. FAS is commonly expressed in a variety of tissues and cells, and is abundantly expressed in mammalian liver, kidney, brain, lungs and mammary glands, as well as adipose tissue.

 

Principle of Assay:

FAS catalyses the production of long-chain fatty acids and NADP+ from acetyl CoA, malonyl CoA and NADPH; NADPH has an absorption peak at 340 nm, whereas NADP+ does not; the rate of decrease in light absorption at 340 nm was measured to calculate FAS activity.

 

Prepare your own instruments and supplies:

Mortar and pestle, ice, benchtop centrifuge, UV spectrophotometer, 1 mL quartz cuvette, adjustable pipette gun and distilled water.

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