| name: |
Rat α-Glucosidase ELISA Kit
|
| Item number: |
ADL-EL-RT00215 |
| Adaptive species: |
Rat |
| Specifications: |
48T/96T |
| storage: |
Store at 2-8 ℃ for 6 months |
| application: |
Ratα-Glucosidase ELISA Kit can quantitatively measure the concentration of testosterone in plasma, tissue homogenates, and other biological fluids in vitro |
| test method : |
Double antibody sandwich method |
| Detection range: |
1.56-100ng/ml |
| sensitivity: |
< 0.938ng/ml |
| standard curve : |
 |
| rate of recovery: |
Add a certain amount of α-glucosidase to the samples listed in the table below, and calculate the recovery rate by comparing the measured value with the expected amount of α-glucosidase in the sample
| |
Recycling scope(%) |
average value(%) |
| Serum(n=5) |
91-102 |
97 |
| EDTA plasma(n=5) |
92-105 |
98 |
| Heparin plasma(n=5) |
88-101 |
96 |
|
| Linearity: |
The linearity of the reagent kit is determined by detecting samples and their series of dilutions mixed with appropriate concentrations of α-glucosidase, expressed as a percentage of the detected concentration relative to the expected concentration
| sample |
1:2 |
1:4 |
1:8 |
| serum(n=5) |
87-102% |
89-105% |
86-96% |
| EDTA plasma(n=5) |
83-95% |
83-97% |
83-98% |
| Heparin plasma(n=5) |
86-98% |
85-98% |
87-98% |
|
| Coefficient of variation(%): |
Within batch difference:CV<8% Batch difference:CV<10% |
| matters needing attention: |
For scientific research purposes only |