Name:Total Cholestenone(TC) Content Assay Kit
Determination method: Micro method
Specification: 100T/96S
Storage conditions: low temperature and light protection
Validity: 6 months
Note: Choose 2-3 samples with large expected differences for pre-calibration before formal determination.
Measurement significance:
TC includes free cholesterol and cholesteryl esters.TC is the total cholesterol contained in all lipoproteins in tissues.
Principle of Assay:
Use esterase to catalyse the hydrolysis of cholesteryl ester to produce free cholesterol (FC) and free fatty acid (FFA), thus converting cholesteryl ester to FC; further use cholesterol oxidase to catalyse the oxidation of FC to produce △4-cholestenone and H2O2; finally, use peroxidase to catalyse the oxidation of 4-aminoantipyrine and phenol by H2O2, resulting in red quinone compounds; there is a characteristic absorption peak at 500 nm. peak at 500nm, and its colour depth is proportional to the TC content.
Bring your own instruments and supplies:
Water bath, adjustable pipette gun, enzyme labeller, 96-well plate.
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