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α-glucosidase(α - GC)Assay Kit (Trace method/48 samples)

Item No.: 00452
Name: α-glucosidase(α - GC)Assay Kit
Determination method: Micro assay
Specification: 100 tubes/48 samples
Storage condition: low temperature and keep away from light
Valid period: 6 months
Description
Description

Name: α-glucosidase(α - GCAssay Kit

Determination method: Micro assay

Specification: 100 tubes/48 samples

Storage condition: low temperature and keep away from light

Valid period: 6 months

Note: 2-3 samples with large expected differences must be taken for pre-calibration before formal measurement.

 

Measurement of significance:

α-GC (EC 3.2.1.20) is widely found in animals, plants, microorganisms and cultured cells, catalysing the hydrolysis of the α-glycosidic bond between an aryl or hydrocarbon group and a sugar group to produce glucose, which is not only related to the relaxation or reinforcement of the cell wall, but also intimately associated with cell recognition and the production of some signalling molecules.

 

Principle of Determination:

α-GC decomposes p-nitrophenyl-α-D-glucopyranoside to produce p-nitrophenol, which has a maximum absorption peak at 400 nm, and the α-GC activity is calculated by determining the rate of increase of absorbance value.

 

Self-contained supplies:

Visible spectrophotometer/enzymometer, benchtop centrifuge, water bath, adjustable pipette, micro quartz cuvette/96-well plate, mortar and pestle, ice and distilled water.

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